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Always run ELISA samples in duplicate or triplicate.

This will provide enough data for statistical validation of the results.

Calculate the average absorbance values for each set of duplicate standards and duplicate samples.We recommend including a standard on each ELISA plate to provide a standard curve for each plate used.A representative standard curve is shown in the figure below, from human HIF1 alpha Simple Step ELISATM kit (ab171577).With this report, you will: Alice is a technical leader in the field of Machine Learning.Her experience spans algorithm and platform development and applications.

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These interventions also are needed if training is to result in sustained new behaviors needed to achieve new performance levels, for an individual, an occupation, or an entire organization.